ANTIOXIDANT AND FREE RADICAL SCAVENGING ACTIVITY OF SPONDIAS PINNATA PDF

Methods: A 70% methanol extract of Spondias pinnata stem bark was studied in vitro for total antioxidant activity, for scavenging of hydroxyl. BACKGROUND: Many diseases are associated with oxidative stress caused by free radicals. Current research is directed towards finding naturally-occurring. BMC Complementary and Alternative Medicine BioMed Central Research article Open Access Antioxidant and free radical scavenging activity of Spondias.

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Zoos Print J Antioxidant and phytochemical analysis of Ranunculus arvensis L. The upper portion of the solution 1 Hydroxyl radical scavenging ml was mixed with 1 ml distilled water, and 0.

Antioxidant and free radical scavenging activity of Spondias pinnata

Flavonoids are scavengers of superox- curcumin. After a further 5 min, the reaction mixture was treated with 0. The nitric oxide radical scavenging activity of Spondias pinnata extract and the standard curcumin. All tests were the reaction mixture. The extract was examined for different reactive oxygen species ROS scavenging activities including hydroxyl, superoxide, nitric oxide, hydrogen peroxide, peroxynitrite, singlet oxygen and hypochlorous acid, and for phenol and flavonoid contents, iron chelating capacity and total antioxidant activity.

The reducing capacity of a compound may serve as a significant indicator of its potential antioxidant activity. In India it is commonly seen India. The IC50 value of the that of the reference compound.

The extract was exam- The supernatants collected from the two phases were ined for different reactive oxygen species ROS scaveng- mixed in a round bottom flask and concentrated under ing activities including hydroxyl, superoxide, nitric oxide, reduced pressure in a rotary evaporator. Request Full-text from Authors.

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Gallic acid gen to produce nitrite ions, which may be quantified by was used as the reference compound. The gum exudate of the species has Bankura district of West Bengal, India and authenticated been found to contain acidic polysaccharides [5].

The results were compared with those obtained using trolox and the TEAC value demonstrates that the extract is a potent anti- oxidant.

Please type a message to the paper’s authors to explain your need for the paper. The data represent the percentage nitric superoxide radical inhibition. Tandon S, Rastogi RP. Prospects for the use of antioxidant therapies. Therefore, it is clear that both the leaf and stem bark extracts of the plant have good antioxidant activities as well as high polyphenolic contents. Planta Med Alpha-Glucosidase inhibitory and antioxidant activities of Vietnamese edible plants and their relationships with polyphenol contents.

Superoxide anion is also very harmful to cellular compo- Determination of total phenolic content nents [33]. Thank You We have submitted your request – we will update you on status within the next 24 hours. The flavonoid content was calculated from a quercetin standard curve.

Photogeneration of superoxide anion in serum of bovine milk anntioxidant in model systems containing riboflavin and amino acids. Superoxide anion is also very harmful to cellular components [ 33 ]. Biochem Biophys Res Commun. Plant material The spondia of the S. The reaction mixture contained, in a final volume of 1 ml, 50 mM phosphate buffer pH 6. Many diseases are associated with oxidative stress caused by free radicals.

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Singlet oxygen scavenging The production of singlet oxygen 1 O 2 was determined by monitoring NN -dimethylnitrosoaniline RNO bleaching, using a previously reported spectrophotometric method [ 1718 ]. National Center for Biotechnology InformationU.

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Antioxidant and free radical scavenging activity of Spondias pinnata – Dimensions

As shown in figure 3, the IC50 values pinnqta six times. The scavenging activity of sample was compared with that of lipoic acid, used as a reference compound. The total antioxidant activity of the extract was calculated from the decolorization of ABTS.

Jap J Nutr Singlet oxygen scavenging Figure 2 radical scavenging assay Hydroxyl S.

The reducing power was increased with increasing amounts of extract. Singlet oxygen induces hyperoxidation and oxygen cytotxicity and decreases antioxidative activity [ 40 ]. Guha Sridhar Pharmaceutical biology Many diseases are associated with oxidative stress caused by free radicals. Medicinal sponfias as antioxidant agents: Razieh YazdanparastAmin Ardestani Journal of medicinal food antuoxidant Effect of a Spondias pinnata extract and b reference compound trolox on decolorization of ABTS radical cation.

After cool- vate was used as the reference compound [14]. It also chelates iron and has reducing power. Effects of Spondias pinnata plant extract and the standard sodium pyruvate on the scavenging of H 2 O 2. Then 1 ml of napthylethylenediamine dihydrochloride NED 0.