Cette biodégradation est basée sur le fonctionnement d’un consortium bactérien complexe, composé de bactéries autotrophes et hétérotrophes, associées sous. Request PDF on ResearchGate | Influence possible des protozoaires sur le taux de mortalité des bactéries autotrophes nitrifiantes | Le modele de l’IAWQ du. Bactérie (Bacteria): Les bactéries sont des organismes vivants unicellulaires, très Les bactéries autotrophes telles que Nitrobacter, active dans les processus.

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Change the order of display of the official languages of Canada English first French first Option to display the non-official languages Spanish or Portuguese Neither Spanish Portuguese Display definitions, contexts, etc. The most frequently used enumeration methods of nitrifying bacteria are not very satisfactory.

Our conclusion is that the results presented in this paper allow the validation of the 14C-bicarbonate incorporation method with and without inhibitors to measure the nitrifying activity. Continuous enrichment cultures of nitrifying bacteria were obtained from an inoculum coming from the Seine estuary freshwater section. Different methods of measuring the nitrifying activity exist.

Download the article in PDF to read it. This factor is considered to be constant in the standard incubation conditions. This factors allowed us to establish a relationship between potential nitrifying activity measurements and nitrifying biomass: By regenerating oxidised forms of nitrogen nitratethe nitrification process plays an important role in the nitrogen cycle of aquatic environments.

Bicarbonate was measured by acid titration in natural water samples, and with the evolution method for culture samples. Pure cultures of Nitrosomonas europaea and Nitrobacter winogradskyi were obtained from the National Collection of Industrial and Marine Bacteria Aberdeen, Scotland and a continuous enrichment culture of mixed heterotrophic bacteria, without nitrifying organisms, was obtained with a freshwater inoculum by imposing a residence time of 2 hours less than the generation time of nitrifying bacteria.


The measurement of the activity and biomass of nitrifying bacteria is bacyerie essential to understand and quantify the general nitrogen fluxes in those environments.

For this reason N-serve was added in the empty flask before the sample to allow the evaporation of the solvent. Access a collection of Canadian resources on qutotrophe aspects of English and French, including quizzes. The first methods developed were based on the use of specific nitrification inhibitors: The general validity of the method was demonstrated by experiments on nitrification inhibitors in enrichment cultures. Biovolumes were estimated according to cell size and converted in biomasses according to a conversion factor determined experimentally with a carbon analyser.

These experiments consisted in measuring the effect of different combinations of N-serve, ethanol the organic solvent of N-serve and chlorate, on Autotropye rates and C incorporation rates on samples of the two nitrifying enrichment cultures ammonium- and nitrite-oxidising bacteria.

Une réévaluation de la methode d’incorporation – Revue des sciences de l’eau – Érudit

A collection of writing tools that cover the many facets of English and French grammar, style and usage. In which subject field? Recently developed gene-probes techniques work well for the identification of particular strains, but are not yet useful for the numeration.

However, the ethanolic solution of N-serve had an unwanted result on C incorporation.

Nitrifying activity, nitrifying biomass, 14C-bicarbonate incorporation, nitrification inhibitors, N-serve, chlorate. FAQ Frequently asked questions Display options. The method can also be used by placing the sample in optimal temperature, pH, oxygen and ammonium conditions for nitrifying bacteria, to allow potential nitrifying activity measurements. Ammonium was measured with the indophenol blue method, nitrate was reduced in nitrite on a cadmium bed and nitrite was measured with the sulfanilamide method.

Glossaries and vocabularies Access Translation Bureau glossaries and vocabularies. Bacteria which are able to multiply by using inorganic matter as the only source of carbon and nitrogen. The method can be used in in situ conditions, to allow real nitrifying activities measurements in samples. To measure potential nitrifying activities, the optimal growth conditions of nitrifying bacteria were determined on enrichment cultures: Revue des sciences de l’eau11 2— Revue des sciences de l’eau 11, no.


Several methods were used for our study: N-serve, allyl thio-urea, acetylene, methylfluoride and dimethyl ether, as most used. A good alternative to these methods consists in the measurement of potential nitrifying activity which is correlated to the nitrifying biomass. C incorporation rates are measured by the incubation of samples with 14C-bicarbonate, the samples being filtered on 0.

The determined optimal growth rate was 0.

This work presents a bactdrie of the autotrophic nitrifying activity measurement by the 14C-bicarbonate incorporation method and its use to estimate the biomass of nitrifying bacteria. The inhibitors effects were also determined on the C incorporation rates of heterotrophic bacteria. Language Portal of Canada Access a collection of Canadian resources on all aspects of English and French, including quizzes. By acting this way, inhibition of autotrophic C incorporation by nitrifying bacteria was also uatotrophe, while heterotrophic incorporation was unaffected.

These methods can be applied as long as the inhibitors are specific for nitrifying bacteria, and activities are high enough to allow the measurement of concentration variations during incubation times which are not too long. The main differences of our protocol to the original ones is that we propose the use of a combination of 2 inhibitors, N-serve and chlorate, and the elimination by evaporation of the organic solvent of N-serve ethanol to avoid any interference with the heterotrophic populations.

Classical culture techniques most probable number and immunofluorescence techniques are known to greatly underestimate the numbers of active organisms.